A 2007 study done by Wildlife International, Ltd.  determined a lactylate's ready biodegradability by the carbon dioxide evolution test method. The study was performed on a LEFA sodium salt produced from oleic acid and lactic acid. The test method determines if microbes, in this case activated sludge inoculum, can digest a test material, thereby returning the carbon-based material back into the environment as carbon dioxide to complete the carbon cycle . To meet or exceed the OECD Guideline 301B criteria for "readily biodegradable",  a sample must produce 60% of the theoretical amount of carbon dioxide (TCO 2 ) within a 10-day window of reaching 10% TCO 2 . The LEFA used in the study had a final average cumulative percent biodegradation of % and the test solution had a pH of at the end of the 28-day test. Therefore, the test material met the criteria to be considered readily biodegradable. In the presence of water, lactylates will break down ( hydrolyze ) into fatty acid and lactic acid .  Based on all available information, lactylates do not meet any hazard categories under SARA Title III, Sections 311–313 .